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Amazoncom dna repair a laboratory manual of research procedures volume 1 part b 9780824711849 errol c friedberg philip c hanawalt books. Skip to main content advertisement. Add 1 g of salmon sperm dna to 100 ml of 04 m naoh and mix to dissolve boil for 30 minutes in a water bath place on ice to cool then cool to room temperature adjust ph to 70 using glacial acetic acid aliquot 15 ml to each of seven 50 ml tubes add two volumes of cold 100 ethanol and store at 20c for at least 1 hour. In dna repair a laboratory manual of research procedures ec friedberg and pc hanalwalt eds vol 1 part b pp 379 401 marcel dekker new york. Official methods of analysissm oma is the most comprehensive and reliable collection of chemical and microbiological methods and consensus standards availablemany official methods have been adopted as harmonized international reference methods by the international organization for standardization iso international dairy federation idf international union of pure and applied chemistry